The principal chemical element of cannabis, cannabidiol ( CBD), has been shown to have antitumor properties. The present study examined the in vitro effects of CBD on human gastric cancer SGC-7901 cells. We identified that CBD substantially inhibited the proliferation and colony formation of SGC-7901 cells. Additional investigation showed that CBD substantially upregulated ataxia telangiectasia-mutated gene (ATM) and p53 protein expression and downregulated p21 protein expression in SGC-7901 cells, which subsequently inhibited the levels of CDK2 and cyclin E, thereby resulting in cell cycle arrest at the G0-G1 phase. In addition, CBD substantially improved Bax expression levels, decreased Bcl-two expression levels and mitochondrial membrane possible, and then upregulated the levels of cleaved caspase-three and cleaved caspase-9, thereby inducing apoptosis in SGC-7901 cells. Lastly, we identified that intracellular reactive oxygen species (ROS) improved following CBD remedy. These outcomes indicated that CBD could induce G0-G1 phase cell cycle arrest and apoptosis by escalating ROS production, top to the inhibition of SGC-7901 cell proliferation, thereby suggesting that CBD could have therapeutic effects on gastric cancer.
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Zhang X1, Qin Y1, Pan Z1, Li M1, Liu X1, Chen X1, Qu Gtwo, Zhou Lthree, Xu Mthree, Zheng Qfour, Li Dfive.